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Abstract Availability of readily transformable germplasm, as well as efficient pipelines for gene discovery are notable bottlenecks in the application of genome editing in potato. To study and introduce traits such as resistance against biotic and abiotic factors, tuber quality traits and self-fertility, model germplasm that is amenable to gene editing and regeneration is needed. Cultivated potato is a heterozygous autotetraploid and its genetic redundancy and complexity makes studying gene function challenging. Genome editing is simpler at the diploid level, with fewer allelic variants to consider. A readily transformable diploid potato would be further complemented by genomic resources that could aid in high throughput functional analysis. The heterozygous Solanum tuberosum Group Phureja clone 1S1 has a high regeneration rate, self-fertility, desirable tuber traits and is amenable to Agrobacterium-mediated transformation. We leveraged its amenability to Agrobacterium-mediated transformation to create a Cas9 constitutively expressing line for use in viral vector-based gene editing. To create a contiguous genome assembly, a homozygous doubled monoploid of 1S1 (DM1S1) was sequenced using 44 Gbp of long reads generated from Oxford Nanopore Technologies (ONT), yielding a 736 Mb assembly that encoded 31,145 protein-coding genes. The final assembly for DM1S1 represents a nearly complete genic space, shown by the presence of 99.6% of the genes in the Benchmarking Universal Single Copy Orthologs (BUSCO) set. Variant analysis with Illumina reads from 1S1 was used to deduce its alternate haplotype. These genetic and genomic resources provide a toolkit for applications of genome editing in both basic and applied research of potato. 

Abstract GW230529 is the first compact binary coalescence detected by the LIGO–Virgo–KAGRA collaboration with at least one component mass confidently in the lower mass gap, corresponding to the range 3–5M_⊙. If interpreted as a neutron star–black hole merger, this event has the most symmetric mass ratio detected so far and therefore has a relatively high probability of producing electromagnetic (EM) emission. However, no EM counterpart has been reported. At the merger timet₀, Swift-BAT and Fermi-GBM together covered 100% of the sky. Performing a targeted search in a time window [t₀− 20 s,t₀+ 20 s], we report no detection by the Swift-BAT and Fermi-GBM instruments. Combining the position-dependentγ-ray flux upper limits and the gravitational-wave posterior distribution of luminosity distance, sky localization, and inclination angle of the binary, we derive constraints on the characteristic luminosity and structure of the jet possibly launched during the merger. Assuming atop-hatjet structure, we exclude at 90% credibility the presence of a jet that has at the same time an on-axis isotropic luminosity ≳10⁴⁸erg s⁻¹in the bolometric band 1 keV–10 MeV and a jet opening angle ≳15°. Similar constraints are derived by testing other assumptions about the jet structure profile. Excluding GRB 170817A, the luminosity upper limits derived here are below the luminosity of any GRB observed so far. 

Abstract Gamma-ray bursts (GRBs) are considered as promising sources of ultra-high-energy cosmic rays (UHECRs) due to their large power output. Observing a neutrino flux from GRBs would offer evidence that GRBs are hadronic accelerators of UHECRs. Previous IceCube analyses, which primarily focused on neutrinos arriving in temporal coincidence with the prompt gamma-rays, found no significant neutrino excess. The four analyses presented in this paper extend the region of interest to 14 days before and after the prompt phase, including generic extended time windows and targeted precursor searches. GRBs were selected between 2011 May and 2018 October to align with the data set of candidate muon-neutrino events observed by IceCube. No evidence of correlation between neutrino events and GRBs was found in these analyses. Limits are set to constrain the contribution of the cosmic GRB population to the diffuse astrophysical neutrino flux observed by IceCube. Prompt neutrino emission from GRBs is limited to ≲1% of the observed diffuse neutrino flux, and emission on timescales up to 10 4 s is constrained to 24% of the total diffuse flux.